The study aimed to identify the intermediate role of knowledge and information management in the relationship between adopting the strategy criterion and improving the overall performance. The study used descriptive analytical method. The study was conducted on the university leadership in Al-Azhar, Islamic and Al-Aqsa Universities. The study population consisted of (416) individuals and sample study consisted of the (200) individuals (182) individuals responded, and the questionnaire was used in the collection of data. The study reached a number of results, the most important of which were: The level of adoption by the Palestinian universities of the strategy criterion was very high. The level of adoption by the Palestinian universities of the knowledge and information management standard was very high. The overall performance level in the Palestinian universities under study was significant. The overall performance of the universities has a statistically significant impact on the adoption of the strategy criterion on knowledge and information management. There is a statistically significant impact of the adoption of knowledge and information management on improving overall performance in Palestinian universities. This information partially mediates the relationship between adopting the strategy standard and improving overall performance in Palestinian universities The study presented a number of recommendations, the most important of which is: Greater attention to the application of the strategy criterion as a basic guide for excellence in universities. Developing information systems in universities and improving the mechanism of information exchange and knowledge. Work on developing the overall performance of universities through adopting international excellence models.